GeM Registration for tender Supply of Dengue Ns1 Ag Elisa Kit Rate To Be Quoted Per Test Basis 1 The Elisa Kit Should B E Designed For Qualitative Detection Of Dengue Ns1 Antigen Of All 4 Dengue Serotypes In Human Serum. 2 The Kit Should Be Provided With The Following Materials And Reagents: A Anti- Ns1 Antibody Coated Breakway Microwell S. Desiccant Should Be Provided For Storing The Unused Microwells Which Are To Be Resealed Immediately. B Hors Eradish Peroxidase Conjugated Anti-Ns1 Monoclonal Antibody With Preservatives C Chromogenic Substrate In Bu Ffer D Positive Control In The Form Of Recombinant Antigen With Preservatives And Antibiotics E Negative Control In The Form Of Confirmed Negative Human Serum With Preservatives And Antibiotics F Calibrators In The Form Of Reco Mbinant Antigen With Preservatives And Antibiotics G Sample Diluents H Wash Buffer 3 The Time Required For Perf Orming The Test For Detection Of Dengue Ns1 Antigen By Elisa Should Range Between 2-4 Hours. 4 The Elisa Kit For Detection Of Dengue Ns1 Antigen Should Have A Sensitivity Of >90% And A Specificity Of >95% Taking Rt-Pcr As The Gold Standard. 5 The Kit Should Have A Shelf-Life Of At Least 18 Months When Stored At An Ambient Temperat Ure Of 2 Degree C - 8 Degreec. 6 Transportation Should Be Under Cold Chain. Item Code S60096, Ai 23-24

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